Immunofluorescent Cell-Counting Assay for Lymphocytic Choriomeningitis Virus
نویسندگان
چکیده
منابع مشابه
Immunofluorescent cell-counting assay for lymphocytic choriomeningitis virus.
A quantitative assay for lymphocytic choriomeningitis virus was developed and standardized. The assay is based on direct immunofluorescent staining of infected L-929 cell monolayers and enumeration of cells containing fluorescent viral antigens. Maximal adsorption of virus to cells occurred within 1 h. Observations on the sequential development of viral antigens within cells showed that specifi...
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LCM virus antigen was visualized in infected tissue cultures and mouse tissues by the direct immunofluorescent procedure. In all instances, antigen was localized to the cytoplasm. In intracerebrally infected mice, antigen was almost completely restricted to meninges, choroid plexus, and ependyma; at no time were neurons involved. Mice infected by intraperitoneal inoculation of a viscerotropic s...
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There has been an upsurge of interest in lymphocytic choriomeningitis virus (LCMV) as a human pathogen and specifically as an underdiagnosed congenital infection (1,2). LCMV is a member of the Arenaviridae family of viruses. Arenaviruses have a complex life cycle involving chronic infection and shedding of the virus in rodents and episodic infections in humans (3,4). Six of the arenaviruses (La...
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A rapid, quantitative assay of interferon was developed, based on the reduction of the number of virus-infected cells by dilutions of interferon. Infected cells were enumerated by fluorescent cell-counting 24 hours after challenge of interferontreated McCoy cover-slip cell monolayers. A quantitative linear relationship between the reduction of fluorescent cells and dilutions of interferon was d...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1974
ISSN: 0003-6919
DOI: 10.1128/am.28.1.17-21.1974